How to Perform and Interpret a Urinalysis Using Wards Simulating Lab Activity
Urinalysis is a common diagnostic test that can provide valuable information about the health status of a person. It involves the physical, chemical, and microscopic examination of urine samples to detect the presence of abnormal substances or indicators of disease. Urinalysis can help diagnose conditions such as urinary tract infections, kidney disorders, diabetes, liver problems, and more.
Wards Simulating Lab Activity is a kit that allows students to perform urinalysis on four simulated urine samples from different patients. The kit contains materials and instructions for testing the pH, sugar, protein, and microscopic components of the urine samples. The kit also provides case studies of the patients and possible diagnoses based on the urinalysis results.
In this article, we will explain how to use the Wards Simulating Lab Activity kit and how to interpret the urinalysis results. We will also provide an answer key for the four simulated urine samples.
The procedure for performing urinalysis using Wards Simulating Lab Activity kit consists of five parts: physical characteristic observations, testing pH, testing for sugar (Benedict's test), testing for protein (Biuret test), and microscopic observations. The following steps describe how to carry out each part:
Physical characteristic observations:
Label four medicine cups 1-4.
Shake each urine sample thoroughly and dispense 10 ml of each sample into the properly labeled medicine cup.
For each urine sample, observe and record the color, clarity, and smell of the urine.
Dip a pH strip into the simulated urine from Patient #1.
Compare the color of the test strip to the comparator chart after 20-30 seconds of sampling. Record the pH in Table 1.
Repeat steps 1 and 2 for the remaining samples.
Testing for sugar (Benedict's test):
Place 250 ml of water into a 400 ml beaker and place the beaker on a hot plate.
To a test tube, add 3 ml of the simulated urine sample from Patient #1 and 3 ml of Benedict's solution.
Record the initial color of the solution before heating in Table 2.
Using a test tube holder, place your test tube in a hot water bath and allow it to boil for 2 minutes. Remove the sample from the hot water bath and record any color change in Table 2.
Repeat steps 1-4 for the remaining samples.
A positive reaction will result in a yellow to red color. Examine your data and note whether it was a positive or negative reaction in Table 2.
Testing for protein (Biuret test):
Remove 3 ml of the simulated urine sample from Patient #1, and place it in a test tube. Record the initial color of the urine in Table 3.
Add 1 ml of Biuret solution to the urine and swirl.
Record the color of the solution in Table 3.
Repeat steps 1-3 for the remaining samples.
A positive test will result in an orange-red color, and a negative reaction will give a green color. Examine your data and note whether it was a positive or negative reaction in Table 3.
Place one drop of simulated urine from Patient #1 on a microscope slide and cover it with a cover slip.
Examine the slide under low power (100x) and high power (400x) magnification using a compound microscope. Look for any cells, crystals, bacteria, or other structures in the urine sample. Sketch what you see in Table 4.